Nuclease Activity in a Fragment of Bacteriophage T4 Deoxyribonucleic Acid Polymerase Induced by the Amber Mutant am B22
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چکیده
منابع مشابه
Nuclease activity in a fragment of bacteriophage T4 deoxyribonucleic acid polymerase induced by the amber mutant am B22.
T4 bacteriophage mutant am B22 (gene 43), an amber mutant in the gene for DNA polymerase, produces a protein which lacks the polymerase but retains the nuclease function of the wild type enzyme. The am B22 nuclease is shown to be a fragment of the wild type polymerase by comparing the tryptic peptides of the two proteins. The wild type and mutant enzymes each consist of a single polypeptide cha...
متن کاملOn the direction of reading of bacteriophage T4 gene 43 (deoxyribonucleic acid polymerase).
Amber (am) mutants of the two closely linked sites, B22 and C125, in bacteriophage T4 gene 43 [deoxyribonucleic acid (DNA) polymerase] synthesize in the nonpermissive (su(-)) Escherichia coli host gene 43 products which are devoid of DNA polymerase activity, but which retain a 3'-exonuclease activity. Diethylaminoethyl-cellulose chromatographic analysis of DNA polymerase and deoxyribonuclease a...
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Ultraviolet irradiation of Escherichia coli polA(-) cells reduces their capacity to support the growth of T4 phage. There is no additional loss of capacity observed in pol tsA(-)recA(-) double mutants at the nonpermissive temperature. The reversion frequency of a T4 rII mutant after ultraviolet irradiation is not changed by the absence of host deoxyribonucleic acid polymerase I.
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Nucleotide pools were analyzed under different conditions of infection by bacteriophage T4, for three reasons: (a) as an approach to identifying the defective functions associated with conditional lethal phage mutants bearing uncharacterized lesions in DNA replication; (b) to gain information about the control of nucleotide synthesis in phage-infected Escherichia coli; and (c) as an aid to inte...
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DNA polymerase induced in Escherichia coli by phage T4 am N82 has been prepared in a highly purified state. The molecular weight of about 112,000 for this enzyme compares with a value of 109,000 for the host (E. coli) DNA polymerase. The amino acid compositions of the two enzymes, however, are distinctive, especially the half-cystine value of 15 residues for the phage enzyme and 3 for the bacte...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1971
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)61923-4